Lipase Activity

Enzyme Activity

Research Question:
Does increasing the temperature of 3ml lipase join on the assess of response in enzyme-catalyzed reply?

Hypothesis:
One of the main conditions that drop arrogate enzyme activity is temperature. The rate of an enzyme-catalyzed reaction increases as the temperature is increased. A 10 degree Celsius rise in temperature will increase the activity of most enzymes by 50-100%. slightlywhat, as small as 1-2 degrees Celsius may change to 10-20% in the results of fluctuation in reaction temperature. In the case of enzymatic reactions, many enzymes are change by high temperatures. At consistent temperatures, enzymes will be deactivated. Storage of enzymes at 5°C or to a lower place is generally the most suitable. Some enzymes lose their activity when frozen. Some enzymes denatured when temperature increases too much, a potentially permanent process.

Variables:

self-sufficient Variable:
Temperature, 0°C, 15°C, 35°C, 45°C, 60°C. This is because it is the only thing we are changing everywhere and over in the lab.

Dependent Variable:
The time taken in seconds the enzyme to react at different temperatures. This is because the temperature of the enzyme is reacting at will either make the enzyme reacts faster or slower.



The rate of digestion will be calculated by 1/t (sec-1)

Controlled Variables:
preoccupancy of enzyme (3 ml lipase)
X nextag shop;
Lipase Concentrate-HP 90 UltraCaps - Integrative Therapeutics; $24
(http://www.nextag.com/enzyme-lipase/compare-html)
concentration of substrate (3 ml cooking oil)
concentration of inhibitor (1 ml freshness salt)
All of these are fixed so that it does not affect the reaction.
Introduction:
Proteins that are made up of long arrange of amino acids are called enzymes. These enzymes act as catalysts in chemical substance reactions which decreases the activation required for the reaction and consequently increasing the rate of reaction. Substrates are those chemicals that are transformed with the help of...If you want to go a full essay, order it on our website: Ordercustompaper.com



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